PUO Profile Test

PUO Profile is used to detect the IgM Antibodies to Dengue, Leptospira, Brucella, EBV infections apart from Aerobic & Anaerobic Blood Culture test.

• The test is done to diagnose and detect the possible underlying infective causes for the pyrexia or persistent fever.
• To rule out the infective causes for the pyrexia
• To diagnose cases of dengue, leptospira, brucella or EBV infections showing atypical presentation or that are asymptomatic clinically.

• Patients with persistent fever for 3 weeks or more
• Immunocompromised patients presenting with atypical symptoms and fever.
• Patients with recent exposure to infections.
• Patients presenting with unknown causes of GI related symptoms like diarrhoea, abdominal cramps, fever etc.

This test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.

No special precautions are required.

Dengue IgM

Positive result indicates the presence of detectable Dengue Virus IgM antibodies which indicates exposure to the dengue virus or the patient is currently infected with dengue virus infections.

Further tests are required to differentiate between primary and secondary infections.

Leptospira Ig M

Detection of leptospira IgM antibodies may indicate a recent infection or recent exposure to antigens (vaccine).

Negative result does not rule out the infection.

Brucella antibodies IgM

Rising titres of antibodies are regarded as serological evidence of recent infection. In the acute stage of the disease there is an initial production of IgM antibodies, followed closely by production of IgG antibodies.

Definitive diagnosis is usually by blood culture or PCR.

EBVIgM antibody

High titres indicate a positive result which shows the presence or exposure to EBV infections.

Further tests and clinical correlation is recommended for definitive diagnosis.

• Aerobic & Anaerobic Blood Culture
• Dengue antibody test
• Leptospira test
• Brucella test
• EBV test

Primary dengue infection is characterised by the presence of significant IgM levels 3 -5 days after the onset of infection, which may persist for 3 -5 months. Secondary infection is characterised by elevation of specific Dengue IgG 1-2 days after the onset of infection and in majority of cases accompanied by elevation of Dengue IgM in early infections.

bacteria like infected soil, water, open wounds. Antibodies appear 7-10 days post infection.

The test is performed to detect antibodies against brucella bacteria, which is a zoonotic bacterium. The infections mainly occur via infected food or dairy products. Antibodies appear after a week post infection.

The test detects the antibody titres to EBV infections.

Apart from these, blood culture tests are also done to detect the culture and sensitivity of aerobic and anaerobic bacteria.

The PUO panel test is used to diagnose and detect possible underlying infective causes for persistent fever (pyrexia of unknown origin, or PUO). It helps rule out infections such as dengue, leptospira, brucella, and EBV.

The PUO Profile Test does not have a "normal range." Instead, it indicates the presence or absence of specific antibodies or bacteria. Positive results suggest an infection, while negative results indicate the absence of the tested pathogens.

Patients with persistent fever for three weeks or more, immunocompromised patients, those with recent exposure to infections, and patients presenting with unknown causes of gastrointestinal-related symptoms are eligible for the PUO Profile Test.

The PUO Profile Test helps diagnose the underlying cause of persistent fever, guides appropriate treatment, and rules out common infectious causes. It provides valuable information for managing patients with pyrexia of unknown origin.

Testing is typically done once the clinical criteria for PUO are met. However, it may be repeated based on clinical judgment and the need for further investigation, as determined by your doctor.

There is no specific time requirement for the PUO Profile Test. It can be done at any time as per your convenience and medical advice from your doctor.

No, fasting is not required for the PUO Profile Test. You can undergo the test without any special dietary preparations.

No special precautions are required other than standard venipuncture techniques. Your healthcare provider will ensure that proper procedures are followed during the blood sample collection process.

The PUO Profile Test includes parameters such as dengue IgM antibodies, leptospira antibodies, brucella antibodies, EBV antibodies, and aerobic & anaerobic blood cultures.

The PUO blood test involves collecting a blood sample using standard venipuncture techniques. A healthcare professional will draw blood from a vein in your arm and send it to the laboratory for analysis.

The PUO Profile Test should be done when a patient presents with persistent fever for three weeks or more without a clear diagnosis or when the doctor suspects an underlying infectious cause based on the patient's symptoms and medical history.

The PUO Profile Test becomes positive when specific antibodies or bacteria are detected in the blood sample. Positive results indicate an infection with the respective pathogens being tested.

The blood sample collection for the PUO Profile Test usually takes only a few minutes. However, the processing and analysis of the sample in the laboratory may take a few days, depending on the diagnostic centre.

The turnaround time for PUO Profile Test reports may vary depending on the diagnostic center. Typically, results are available within 24 hours depending on the day/time when the sample is collected and processed. Contact your diagnostic center for specific timelines.