PUO Profile Test

PUO Profile is used to detect the IgM Antibodies to Dengue, Leptospira, Brucella, EBV infections apart from Aerobic & Anaerobic Blood Culture test.

• The test is done to diagnose and detect the possible underlying infective causes for the pyrexia or persistent fever.
• To rule out the infective causes for the pyrexia
• To diagnose cases of dengue, leptospira, brucella or EBV infections showing atypical presentation or that are asymptomatic clinically.

• Patients with persistent fever for 3 weeks or more
• Immunocompromised patients presenting with atypical symptoms and fever.
• Patients with recent exposure to infections.
• Patients presenting with unknown causes of GI related symptoms like diarrhoea, abdominal cramps, fever etc.

This test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.

No special precautions are required.

Dengue IgM

Positive result indicates the presence of detectable Dengue Virus IgM antibodies which indicates exposure to the dengue virus or the patient is currently infected with dengue virus infections.

Further tests are required to differentiate between primary and secondary infections.

Leptospira Ig M

Detection of leptospira IgM antibodies may indicate a recent infection or recent exposure to antigens (vaccine).

Negative result does not rule out the infection.

Brucella antibodies IgM

Rising titres of antibodies are regarded as serological evidence of recent infection. In the acute stage of the disease there is an initial production of IgM antibodies, followed closely by production of IgG antibodies.

Definitive diagnosis is usually by blood culture or PCR.

EBVIgM antibody

High titres indicate a positive result which shows the presence or exposure to EBV infections.

Further tests and clinical correlation is recommended for definitive diagnosis.

• Aerobic & Anaerobic Blood Culture
• Dengue antibody test
• Leptospira test
• Brucella test
• EBV test

Primary dengue infection is characterised by the presence of significant IgM levels 3 -5 days after the onset of infection, which may persist for 3 -5 months. Secondary infection is characterised by elevation of specific Dengue IgG 1-2 days after the onset of infection and in majority of cases accompanied by elevation of Dengue IgM in early infections.

bacteria like infected soil, water, open wounds. Antibodies appear 7-10 days post infection.

The test is performed to detect antibodies against brucella bacteria, which is a zoonotic bacterium. The infections mainly occur via infected food or dairy products. Antibodies appear after a week post infection.

The test detects the antibody titres to EBV infections.

Apart from these, blood culture tests are also done to detect the culture and sensitivity of aerobic and anaerobic bacteria.