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Maternal Screen (Triple Test) Second Trimester Test in Hadapsar

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Test Overview

Triple marker test is basically a screening blood test that is used to screen pregnant women for potential congenital defects in foetus i.e., neural tube defects, Down syndrome and Trisomy 18 in the unborn child. Primarily three hormones are measured in mother’s blood (thus known as the Triple Marker test). This test can be performed any time between 14 to 22.6 weeks of pregnancy. 

This test only determines the possibility that the fetus will have chromosomal abnormalities. This is because it is simply a predictive test. It is not a conclusive test. The triple marker test is named as such because it looks for alpha-fetoprotein, beta-hCG, and unconjugated estriol.

The triple marker test is essentially a blood screening procedure. It is used to check pregnant women for possible congenital problems in the fetus. These could be neural tube defects, Down syndrome, and Trisomy 18. The mother's blood is mostly tested for three hormones (thus known as the Triple Marker test). The pregnancy can be between 14 and 22.6 weeks at the time of this test.

Written by: Dr. Jasmin Surana, MD Biochemistry, Dept. of Biochemistry

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Maternal Screen (Triple Test) Second Trimester Test Price in Pune

Metropolis Healthcare is a leading diagnostics centre and pathology lab in India equipped with the latest state-of-the-art technologies that provides the Maternal Screen (Triple Test) Second Trimester Test with a clear pricing structure

The price of Maternal Screen (Triple Test) Second Trimester Test in Pune is ₹ 2,625

We are committed to deliver accurate and quality results from the best labs in India with complete transparency regarding test cost and turnaround time. No matter where you are, we strive to offer patients high-quality service that is affordable and accessible.

Frequently Asked Questions

Triple marker tests identify pregnancies that have a high risk of babies having the following abnormalities:

  • Trisomy 21 (Down’s syndrome)
  • Trisomy 18 (Edward syndrome)
  • Neural tube defects


These tests are NOT diagnostic tests. Your doctor will order a confirmatory test when the screening test is positive (high risk)

This test is conducted between the 14th and 18th weeks of pregnancy.
The test is often most recommended under the following scenarios

  • Age of the pregnant woman is above 35
  • Dual Marker tests were abnormal
  • Birth defects in the family
  • The pregnant woman is diabetic and under insulin medication
  • If it is suspected that the pregnant woman was exposed to a high dose of radiation
  • A viral infection during pregnancy

AFP (Alpha-fetoprotein) AFP or alpha fetoprotein is made by the foetal liver and some amount of this substance gets into the mother’s blood. Higher levels of AFP are seen in Neural tube defects and levels are decreased in Down syndrome and Edward syndrome. What if I am a man and have high AFP level? What if I am a non-pregnant woman and have high AFP level? In men, non-pregnant women, and children, AFP in the blood can mean certain types of cancer, especially cancer of the testicles, ovaries, stomach, pancreas, or liver are present. uE3 (Unconjugated Estriol) Estriol is a hormone produced by the placenta, using ingredients made by the fetal liver and adrenal glands. In pregnancies with Down Syndrome or Edward Syndrome, it is observed that the Estriol levels are reduced.

How it Works?

All our phlebotomists are vaccinated

At Metropolis Healthcare, your well-being is our top priority. Rest assured, all our skilled phlebotomists are vaccinated, ensuring a safe and secure experience for you. Our team upholds the highest healthcare standards, and your health is in capable hands. Your safety is non-negotiable, and at Metropolis, we take every measure to guarantee it.

Our Phlebotomists maintain a high standard of safety precautions

Our dedicated team of phlebotomists prioritises your safety before anything else. They implement stringent safety measures to guarantee your well-being during their visit.

Strictly adhering to aseptic techniques, our phlebotomists maintain a sterile environment with thorough sanitization and the use of gloves, masks and face shields. Needles are disposed of with utmost care, following rigorous guidelines to minimise any potential risks. We leave no room for compromise when it comes to personal protective equipment (PPE), ensuring both you and our staff are well protected.

Our phlebotomists collect samples safely

The phlebotomists at Metropolis Healthcare are proficient in safe sample collection. They skillfully and gently draw blood, minimizing any discomfort you may feel. We prioritize your safety by using sterile, single-use needles and barcoded vials to completely eliminate any risk of cross-contamination. Our collection area is carefully sanitized before, during, and after your sample collection.

Samples are processed at high-tech facilities

Metropolis Healthcare takes pride in its state-of-the-art facilities, where your samples are thoroughly processed. Our labs are equipped with cutting-edge machinery with advanced technology, eliminating chances of human error. From the moment your sample arrives at our facility, it undergoes an automated process.

Your samples are stored in clean and secure facilities

We leave no room for compromise when it comes to the storage of your samples. Our storage facilities are regularly cleaned and disinfected, upholding the highest standards of healthcare safety.

Temperature-controlled environments and cooling facilities ensure that your samples remain fresh and the results produced are accurate. Our cutting-edge security measures prevent unauthorized access, guaranteeing the confidentiality of your health data.

Metropolis provides accurate digital reports

Our advanced sample processing machines not only deliver swift results to you but also generate precise digital reports. We provide conclusive diagnoses through meticulous re-checks and repeat specimen analyses, if required, at no extra cost, adding an extra layer of accuracy and dependability. This unique approach sets us apart, earning the trust of top doctors and hospitals across the country.

Your health is our priority, and we spare no effort in our commitment to providing you with the most accurate insights you can rely on.

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Why Metropolis?

Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.

We offer a comprehensive range of 4000+ clinical laboratory tests and profiles, which are used for prediction, early detection, diagnostic screening, confirmation and/or monitoring of the disease.

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