Leukemia Comprehensive Workup Panel Test-Acute Leukemia, Bone Marrow

The Leukemia Comprehensive workup panel -Acute Leukemia, Bone Marrow consists of morphological examination of heparin bone marrow sample followed by cytogenetic analysis to detect translocations and genetic mutations in AML and ALL cases using FISH.

Leukemia may often be asymptomatic in the early stages. People with following clinical signs should however, undergo the test:

• Fatigue, easy tiredness
• Unexplained weight loss
• Frequent infections, fever
• Bone or joint pain and tenderness
• Easy bleeding or bruising with red spots or purplish discoloration.

• In case of abnormalities detected in the routine blood examination (CBC) along with other investigations, the Leukemia comprehensive panel may be useful for further diagnosis.
• The test is done in patients in whom acute leukaemia or lymphoma is suspected.
• To find out the type of Acute leukaemia (AML or ALL) based on the blood studies.
• This panel aids proper diagnosis, helps in choosing the right treatment line and monitoring of the patient.

This test requires bone marrow sample along with whole blood smear. A small incision is made on the skin, and a hollow needle is inserted from the bone up to the bone marrow to collect the bone marrow sample. The skin is disinfected before needle insertion and the sample is collected in vacutainer.

Medical history / Heamato-pathological reports is required.

The immunophenotyping of leukemic cells in bone marrow shows differential count of various blood cells.

Based on the differential count percentage, diagnosis of leukemia and its type can be made.

The positivity percentage of Lymphoid T cells, Lymphoid B cells, Myeloid cells and Monocytic cells is further indicative of the Leukemia diagnosis and its respective classification.eg. B - Cell Acute Lymphoblastic Leukemia, Acute Myeloid Leukemia.

Leukemia Translocation Panel (AML)

Positive result may show translocation in INV 16 (P13;Q22), T(15;17) (Q21;Q22), T(8;21) (Q22;Q22) genes. Depending on the translocation type, the prognosis can be determined.

Leukemia panel by FISH - ALL, Leukemic Blood

Positive or abnormal results show rearrangements or mutations in E2A , TEL/AML1 ES, BCR/ABL or MLL genes.

Further correlation with clinical findings, bone marrow studies and cytogenetics studies should also be done.

Based on the results, the prognosis can be determined.

Morphological examination of bone marrow for abnormalities (Differential count, blasts, neutrophils, lymphocytes, eosinophils percentage), RBC structure and platelets examination is done.

The leukaemia cells may continue to multiply and develop into Lymphoid T cells, Lymphoid B cells, Myeloid and Monocytic cells.

The test is useful to detect translocation mutations in chromosomes INV 16 (P13;Q22), T(15;17) (Q21;Q22), T(8;21) (Q22;Q22).

The test is used for the detection of certain genetic mutations in ALL cases using FISH.

• Leukemia Test
• Blood Cancer test
• Acute Leukemia Test