HBV DNA Detection Test by Taqman RT-PCR (Qualitative)

HBV DNA PCR assay is valuable in the:

• Early diagnosis of HBV infection. HBV DNA can be detected 21 days before the appearance of HbsAg
• Indicators of chronic hepatitis
• Demonstrate viral replication in patients with mutant HBV
• Re-emergence of HBV DNA in serum (after it had become non-detectable) following treatment is suggestive of drug resistance

The HBV DNA Detection Test by Taqman RT PCR (Qualitative) measures the presence, not the amount, of Hepatitis B viral DNA in your blood. This highly sensitive test uses a method called Taqman RT PCR to amplify even tiny amounts of the virus's genetic material. By detecting HBV DNA, it helps diagnose an active Hepatitis B infection, which can damage the liver.

HBV test requires a blood sample. A tourniquet (elastic) band is placed tightly on the upper arm. The patient is then asked to make a fist. This helps in the build-up of blood filling the veins. The skin is disinfected before needle insertion and the blood sample is collected in vacutainer.

An abnormal HBV DNA Detection Test by Taqman RT PCR (Qualitative) result, indicating the presence of Hepatitis B viral DNA, suggests a current Hepatitis B infection. While this doesn't necessarily mean severe illness, it necessitates further evaluation by your doctor. They might order additional tests to assess the severity of the infection and liver function.

People with the following symptoms should do the HBV DNA Detection by TaqMan RT PCR test:

1. Pain in the abdomen
2. The darker color of urine than usual
3. Weakness and getting tired easily
4. Fever
5. Pain in the joints
6. Loss of appetite
7. Jaundice
8. Nausea or Vomiting

In the "immune tolerant" stage, children and adults can have viral loads in the millions.

Most children and adolescents who screen positive for the hepatitis B "e" antigen (HBeAg) have high viral loads, but doctors rarely treat patients at this stage. Their viral loads begin to decrease and liver enzymes (ALT/AST) normalize once their immune systems eliminate HBeAg and produce "e" antibodies (HBeAb).

Adults in the "inactive" stage have untraceable or low viral loads and no indications of liver damage.

Adults with normal ALT (SGPT) levels, which generally indicate that there is no current liver inflammatory condition, and unidentifiable or viral loads less than 2,000 IU/mL are usually not treated. However, it is critical to check with your doctor to ensure that there is no indication of advanced liver disease. This stage could last a lifetime, a few decades, or not at all.

People who are in the "active" stage, have elevated viral loads, and show signs of liver damage, require treatment.

These could be people who are HBeAg positive but cannot seroconvert, lose HBeAg, and regain the antibody without significant liver damage. There could be a trend of SGPT/ALT elevation that fluctuates over time without a sufficient immune reaction to seroconvert. This can be risky, causing liver damage, so regular monitoring is essential.

HBV DNA PCR quantitative is a laboratory test that uses polymerase chain reaction (PCR) technology to measure the amount of hepatitis B virus (HBV) DNA in a blood sample. The test provides a quantitative measurement of the viral load, which is useful for monitoring disease progression and determining the effectiveness of antiviral therapy.

The turnaround time for TaqMan RT PCR for HBV DNA detection varies depending on the laboratory performing the test, but results can typically be obtained within 24 hours.

Several factors can affect the results of TaqMan RT PCR for HBV DNA detection, including the quality of the blood sample, the timing of the sample collection, and the presence of inhibitors or contaminants in the sample.

The first marker to appear in the serum of patients with hepatitis B virus (HBV) infection is the hepatitis B surface antigen (HBsAg). It can be detected as early as 1-4 weeks after exposure to the virus and can remain detectable for several months, even after the acute infection has resolved. Therefore, the presence of HBsAg is an important diagnostic marker for acute and chronic HBV infection

• HBV DNA Real-Time PCR
• Hepatitis B Virus DNA Test
• HBV DNA Test